3D Innervated skin Model
3D innervated skin model for assesment of peripheral neurotoxicity in a 96-well high throughput format.
Alcyomics® has developed a 3D tissue model based in an inert polyHIPE scaffold that enables the culture of neuronal and skin cell lineages together in one system. We have developed a unique co-culture system which is capable of including Schwann cells, neurons, fibroblasts and keratinocytes in physiologically relevant structures (Figure 1).
- iPSC derived neurons and Schwann cells are cultured within a fully humanised tissue model including human dermal fibroblasts, ECM proteins and a well defined keratinocyte epidermal layer.
- When exposed to known neuronal irritants, our model is shown to respond as shown though changes in detectable neurotransmitter (Calcitonin gene-related peptide (CGRP) & Substance P) release (Figure 2).
- Markers for mature neurite formation (Beta Tubulin III (TUJ1)) can be observed extending throughout the 3D construct (Figure 3).
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