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Wound healing
Cell Culturing 2D Platform
Alcyomics offers analysis of wound healing using standardised scratch assays with immortalised keratinocytes. In a 2D monolayer in culture plates, a scratch is created. Closure of the scratch over the next 24 hours is imaged by brightfield microscopy (Figure 1A) then quantified by ImageJ (Figure 1B).
Supernatants can be collected for wound healing-associated cytokine analysis by MesoScale Discovery electrochemiluminescence.
Supernatants can be collected for wound healing-associated cytokine analysis by MesoScale Discovery electrochemiluminescence.
Benefits at Alcyomics:
First to market to provide a flexible and comprehensive wound healing assay with expert analysis
Potential to combine our wound healing assays with our portfolio of 3D skin models to suit your research needs
Proven customer satisfaction in this product
Figure 1A. Wound healing assay using a monolayer of keratinocytes.
- 0% serum induces 80% wound closure by 12 hours.
- 1% serum induces 60% wound closure by 12 hours.
- 0.05% Triton-X inhibits scratch recovery.
Figure 1B. Quantification of the percentage of wound remaining
