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Antibody Drug Conjugates
Photosensitivity assessment of Antibody Drug Conjugates
Benefits of the Skimune® assay
Detection of clinically relevant celluar immune responses
Validated in a number of case studies
Available in combination with complementary assaysNon-animal system
For more information on Skimune® assays and how they can help your business please contact us.
Get in touchCase Study:
Our client was involved in the development of a panel of antibody drug conjugates. In addition to the standard Skimune® skin damage histopathology readouts, the client was interested in the effect of these compounds on melanin production. In response, we modified our flagship Skimune® assay to deliver a UV-dependent melanin readout.
Using a semi-automated image analysis system, melanin signals were quantified from H&E images. The results demonstrated increased melanin production as well as increased damage in the skin due to the client compounds.
Untreated control
Antibody drug conjugate treated image
Figure 1: Analysis of compound X after three days in UV-irradiated skin explant
Skimune® was used to investigate the effect of compound X after UV-IR treatment and 3-day incubation. The samples were stained by haematoxylin and eosin (top), then processed to enhance contrast of the melanin signals. The treated samples clearly show higher melanin production, and the melanin signals were quantified.
Skimune® was used to investigate the effect of compound X after UV-IR treatment and 3-day incubation. The samples were stained by haematoxylin and eosin (top), then processed to enhance contrast of the melanin signals. The treated samples clearly show higher melanin production, and the melanin signals were quantified.
Samples which were exposed to UV with the non-addition (negative control) of the test compound 2 showed a low presence of melanin.
A dose effect response could be observed following exposure to test compound 2 at 10nM and 1nM concentration.
A dose effect response could be observed following exposure to test compound 2 at 10nM and 1nM concentration.
A significant increase in the presence of melanin was observed in response to 10nM of test compound 2 in synergy with UV exposure when compared to the 1nM concentration and the non-addition of the compound.
